Skip to main content
. 2022 Nov 15;13:1041099. doi: 10.3389/fneur.2022.1041099

Figure 4.

Figure 4

The effect of TH on structural damage to the TJs after SCI. (A) Representative images of the gaps in the TJs (white arrow). Scale bar: 20 μm. (B) Fluorescence intensity decline of the gap in Figure 1A. Fluorescence intensity declines of 70% were identified as a gap. (C) Representative images of immunofluorescence staining of TJs 4 h post-SCI. Scale bar: 20 μm. (D) The density of the gaps that emerged on claudin-5 with TH 4 h post-SCI (n = 5 per group, total 153 vessels, Ctrl & NT: P < 0.0001, NT & TH: P = 0.415). (E) The density of gaps that emerged on occludin with TH 4 h post-SCI (n = 5 per group, total 152 vessels, Ctrl & NT: P < 0.0001, NT & TH: P > 0.99). (F) The density of gaps that emerged on ZO-1 with TH 4 h post-SCI (n = 5 mice per group, total 157 vessels, Ctrl & NT: P < 0.0001, NT & TH: P = 0.609). Data are shown as mean ± SEM; ns, non-significance; ****P < 0.0001; nested, one-way ANOVA with Bonferroni's post hoc test.