SDS-PAGE analysis of the interaction of immobilized
recombinant (His)6GlySer-H46A streptokinase with human
plasminogen (A) and of recombinant (His)6GlySer-87-542-W
streptokinase with equine (B), human (C), porcine (D), bovine (E), and
rabbit (F) plasminogen over time (in minutes). (A) HPg, human
plasminogen; HSk, H46A streptokinase; HPsH, plasmin heavy
chain; HPsL, plasmin light chain; HSk*, altered H46A
streptokinase (40.998 kDa). (B) EPg, equine plasminogen; ESk, 87-542-W
streptokinase; EPsH, equine plasmin heavy chain;
EPsL, equine plasmin light chain; ESk*, altered 87-542-W
streptokinase (27.173 kDa). (C) HPg, human plasminogen; ESk, 87-542-W
streptokinase. (D) PPg, porcine plasminogen; ESk, 87-542-W
streptokinase. (E) BPg, bovine plasminogen; ESk, 87-542-W
streptokinase. (F) RPg, rabbit plasminogen; ESk, 87-542-W
streptokinase.