Table 3.
Comparison of the N-gene targeting SARS-CoV-2 qPCR and ddPCR assays.
| Dilution factor | 2019-nCoV_N1 | 2019-nCoV_N2 | NIID_2019-nCoV_N | WH-NIC N | HKU-N | |||||
|---|---|---|---|---|---|---|---|---|---|---|
|
| ||||||||||
| qPCR | ddPCR | qPCR | ddPCR | qPCR | ddPCR | qPCR | ddPCR | qPCR | ddPCR | |
| 10-2 | 18.89 | S* | 18.87 | S | 18.14 | S | 21.40 | S | 20.79 | S |
| 10-3 | 22.16 | 21173 | 22.19 | 20340 | 21.63 | 15173 | 24.65 | 19993 | 24.31 | 18473 |
| 10-4 | 25.99 | 1907 | 26.06 | 1659 | 25.40 | 1177 | 28.52 | 1599 | 28.09 | 1553 |
| 10-5 | 30.66 | 135 | 30.60 | 132 | 29.93 | 60 | 33.11 | 105 | 32.68 | 105 |
| 10-6 | 34.12 | 11 | 34.57 | 8 | 34.91 | 7 | 36.71 | 5 | 36.71 | 12 |
| R2 | 0.9968 | 0.9973 | 0.9943 | 0.9971 | 0.9978 | |||||
*Saturated
**All template were performed in triplicate and data in table indicates average value.