Table 4.
Comparison of the N-gene targeting SARS-CoV-2 qPCR and ddPCR assays on negative patient sample.
Primer-Probes | 2019-nCoV_N2 primer-probe set | WH-NIC N primer-probe set | ||
---|---|---|---|---|
| ||||
Template | qPCR (Ct) | ddPCR (copy/μl) | qPCR (Ct) | ddPCR (copy/μl) |
N1 (153ng/μl) | Undetermined | 2.8 | Undetermined | 0 |
N3 (101ng/μl) | Undetermined | 4.3 | Undetermined | 0 |
N5 (243ng/μl) | Undetermined | 0.5 | Undetermined | 0.5 |
*All template were performed in triplicate and data in table indicates average value.