Table 5.

Results of the Ct values by qPCR and copy number by ddPCR on patient sample.

Primer-probe	2019-nCoV_N2 primer-probe set
Template	P1		P2		P3		P4		P5
Dilution factor	qPCR (Ct)	ddPCR (copy/μl)	qPCR (Ct)	ddPCR (copy/μl)	qPCR (Ct)	ddPCR (copy/μl)	qPCR (Ct)	ddPCR (copy/μl)	qPCR (Ct)	ddPCR (copy/μl)
10-1	26.87	1051	35.03	7.2	35.15	2.4	Undetermined	0	35.05	7.53
10-2	30.52	82	37.03	1.33	Undetermined	0.73	Undetermined	0.53	Undetermined	1.2
10-3	33.81	5.93	Undetermined	0.67	Undetermined	0.6	Undetermined	0	Undetermined	1.07
Primer-probe	WH-NIC N primer-probe set
Template	P1		P2		P3		P4		P5
Dilution factor	qPCR (Ct)	ddPCR (copy/μl)	qPCR (Ct)	ddPCR (copy/μl)	qPCR (Ct)	ddPCR (copy/μl)	qPCR (Ct)	ddPCR (copy/μl)	qPCR (Ct)	ddPCR (copy/μl)
10-1	28.77	836.00	36.11	5	35.88	6.80	Undetermined	1.40	35.86	7.67
10-2	32.25	107.33	38.54	3	38.52	1.93	Undetermined	0.00	37.02	1.87
10-3	35.95	10.40	Undetermined	2.40	38.27	2.07	38.44	1.27	Undetermined	0.47

*The initial concentration of RNA templates were 46.9, 21.1, 27.5, 17.2, and 15.9 ng/μl, respectively.

**All template were performed in triplicate and data in table indicates average value.

⁺Only one of the triplicate was positive (single droplet)