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. 2021 Sep 10;31(12):1732–1740. doi: 10.4014/jmb.2106.06075

Fig. 2. Production of the constructed TEVp variants in the BL21(DE3) (A), BL21(DE3)pLysS (B), Rosetta (DE3) (C) and Origami(DE3) (D) strains.

Fig. 2

About 10 μg of soluble proteins, and 4 μg of proteins in pellets were separated by SDS-PAGE. In the Fig. 2A, the specific bands detected by using anti-His6 monoclonal antibodies for Western blot analysis were shown on bottom of the gel. M: protein marker. S: soluble. P: pellet. Variant names are denoted on the top of the gel. Arrows indicated the position of the TEVp constructs.