Fig. 3. Soluble expression levels of the TEVp constructs in the BL21(DE3) (A), BL21(DE3)pLysS (B), Rosetta (DE3) (C) and Origami(DE3) (D) strains determined by using the EmGFP reporter.

At least five samples were measured under the same induction conditions, and the closest values were counted and indicative of means and standard deviation. The asterisk indicated significant differences higher than the TEVp^5M as the control; * p < 0.01.