Fig. 4. The coupled assay of the cleavage activity of the TEVp^5M or the variant produced in the BL21(DE3) cytoplasm (A), BL21(DE3)pLysS cells (B), Rosetta(DE3) host (C) and Origami(DE3) strain (D).

Data are means and standard deviation of three technical replicates. The heat-inactivated TEVp construct was used as control, and the absorption was subtracted. The asterisk indicated significant differences higher than the TEVp^5M as the control; * p < 0.01.