Table 2.
qRT-PCR analysis of porin genes in individual porin mutants.
| AFS-I- | ||||
|---|---|---|---|---|
|
| ||||
| alr0834 | alr2231 | all4499 | alr4741 | |
|
| ||||
| -ΔΔCt | ||||
| alr0834 | n.d. | 1.8 ± 0.4 | 4.4 ± 0.5 | 0.9 ± 0.4 |
| alr2231 | 0.7 ± 0.3 | n.d. | 0.9 ± 0.5 | 2.8 ± 0.5 |
| all4499 | -0.2 ± 0.1 | 3.3 ± 0.4 | n.d. | 0.2 ± 0.2 |
| alr4550 | -1.5 ± 0.2 | 2.0 ± 0.4 | 0.7 ± 0.3 | -0.3 ± 0.1 |
| alr4741 | -0.1 ± 0.2 | 2.5 ± 0.3 | 0.0 ± 0.5 | n.d. |
| all5191 | 1.0 ± 0.1 | 2.2 ± 0.3 | 0.7 ± 0.4 | 1.3 ± 0.3 |
| all7614 | -2.5 ± 0.1 | 1.6 ± 0.4 | 0.5 ± 0.2 | 0.1 ± 0.2 |
The -ΔΔCt based on the rnpB expression and BG11 values are shown for the mutants. Values in bold are changes with p < 0.05 (Student’s t-test) compared to wild type.