Fig. 4. Results of the MIC test performed by treating constructed E. coli strains with kanamycin, gentamicin and apramycin.

(A) Kanamycin MIC result of E. coli strains after 24 h cultivation at 30°C in the presence of 100 μg/ml ampicillin and 0.05 mM IPTG. Paper discs contained 0, 1, 2, 4, 6, 8, and 10 μg kanamycin. (B) Gentamicin MIC result of E. coli strains under the same conditions as (A). (C) Apramycin MIC result of E. coli strains under the same conditions as (A).