. 2021 Jan 22;31(3):447–455. doi: 10.4014/jmb.2012.12051

Table 2.

Free amino acid profiles in soybean cultures of four Bacillus spp. strains at days 1, 15, and 30. (unit: mg/kg)

Amino acid	Control			B. licheniformis 14ADL4			B. sonorensis SRCM101395			B. subtilis ATCC 21228			B. velezensis JJ-D34

	Day 1	Day 15	Day 30	Day 1	Day 15	Day 30	Day 1	Day 15	Day 30	Day 1	Day 15	Day 30	Day 1	Day 15	Day 30
Neutral amino acid
Alanine	160.67^a	169.17^a	191.52^a	167.67^a	2433.26^bc	3049.71^cd	165.75^a	2034.47^b	2382.13^bc	158.93^a	3388.02^d	3799.28^d	167.77^a	752.88^a	937.89^a
Asparagine	147.08^abc	145.28^abc	147.88^abc	149.68^abc	243.65^bcd	285.11^cd	141.79^abc	62.36^ab	26.05^a	127.47^abc	522.64^e	770.49^f	143.57^abc	245.25^bcd	392.10^de
Glycine	46.11^a	50.93^a	48.09^a	52.93^a	1172.65^d	1519.46^e	50.77^a	757.26^bc	960.31^cd	53.39^a	1970.79^f	2065.46^f	57.75^a	494.35^b	690.44^bc
Isoleucine	41.21^a	42.19^a	50.69^a	46.79^a	2767.39^cd	3318.77^d	47.77^a	1913.29^b	2386.80^bc	57.67^a	3155.71^cd	3562.05^d	64.04^a	3113.75^cd	3284.90^d
Leucine	55.26^a	55.82^a	67.84^a	74.86^a	5695.12^cd	6756.67^de	73.82^a	4159.09^b	4858.47^bc	85.40^a	6710.01^de	7463.43^e	102.82^a	5299.35^bc	5494.63^cd
Methionine	20.21^a	19.35^a	21.99^a	28.04^a	1150.18^def	1191.66^ef	28.80^a	860.04^cd	790.27^bc	31.21^a	1286.70^f	950.86^cde	32.97^a	1030.11^cdef	526.32^b
Phenylalanine	79.03^a	81.29^a	93.30^a	93.67^a	4637.11^cd	5103.84^d	92.25^a	3540.35^b	3731.90^bc	107.18^a	5339.07^d	5745.45^d	119.31^a	5349.07^d	5285.23^d
Proline	56.90^a	32.72^a	33.61^a	31.88^a	2346.05^b	2958.76^bc	31.20^a	2947.26^bc	3079.49^bc	47.95^a	4016.13^bc	4227.06^c	58.32^a	3167.93^bc	3739.25^bc
Serine	51.44^a	52.83^a	61.08^a	57.62^a	1524.70^e	2006.15^f	57.23^a	1165.90^d	1468.59^e	74.70^a	753.50^c	1121.47^d	77.51^a	398.49^b	690.07^c
Threonine	45.51^a	49.16^a	60.11^a	53.44^a	2411.26^f	2434.95^f	50.72^a	1140.35^de	1340.80^e	71.26^a	886.79^cd	1092.44^de	75.19^a	434.53^b	648.53^bc
Tryptophan	176.27^a	180.59^a	190.18^a	194.04^a	1224.23^cd	1325.69^de	196.15^a	763.09^b	832.84^bc	191.29^a	1825.04^f	1732.31^ef	208.42^a	1672.99^ef	1304.24^de
Tyrosine	63.91^a	65.23^a	80.33^a	73.41^a	3213.56^cd	3280.44^cd	70.59^a	2439.95^b	2628.89^bc	89.61^a	3681.09^d	3218.17^cd	97.86^a	2993.13^bcd	2982.32^bcd
Valine	54.58^a	56.74^a	65.65^a	65.66^a	3410.67^bcd	3977.86^cde	66.21^a	2526.52^b	2988.66^bc	78.99^a	4482.40^de	5062.84^e	89.25^a	4398.59^de	4648.09^e
Acidic amino acid
Aspartic acid	53.01^a	56.12^a	60.18^a	60.78^a	2803.34^d	3665.16^e	59.34^a	1924.10^bc	2601.13^cd	78.39^a	3158.57^de	3700.52^e	92.02^a	1220.74^b	1703.74^b
Glutamic acid	404.74^a	410.35^a	434.01^a	420.44^a	18077.65^e	20053.26^e	415.27^a	10107.50^c	11267.42^c	445.43^a	12783.35^cd	14715.05^d	472.16^a	5457.30^b	6723.10^b
Basic amino acid
Arginine	1046.67^d	1059.49^d	1070.95^d	1116.34^d	19.01^a	17.62^a	1122.74^d	32.39^a	35.29^a	1039.68^d	293.31^bc	278.52^bc	1139.26^d	97.20^ab	371.46^c
Histidine	53.06^a	54.18^a	60.52^a	71.27^a	2107.06^d	1990.60^cd	57.10^a	1619.26^bc	1290.84^b	63.51a^a	2136.37^d	1968.19^cd	74.30^a	2065.33^cd	1599.05^bc
Lysine	73.71^a	85.49^a	95.88^a	93.60^a	5057.28^cd	6007.25^de	108.06^a	3823.86^b	4168.82^bc	122.86^a	6921.71^ef	7272.85^f	134.86^a	4945.31^bcd	4509.57^bc
Functional amino acid
GABA	232.27^b	257.63^b	285.51^b	273.72^b	40.36^a	49.14^a	275.59^b	53.89^a	60.93^a	303.79^b	270.59^b	309.81^b	316.22^b	668.28^c	698.54^c

Different superscripts within a row denote a significant difference between mean values (p < 0.05) according to Duncan’s multiple range test.