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. 2020 Dec 31;31(2):226–232. doi: 10.4014/jmb.2008.08048

Fig. 2. SFTSV NSs sequester RIG-I and TBK1 in NSs-induced inclusion bodies (IBs) and inhibit expression of RIG-I and downstream molecules in response to poly I:C treatment.

Fig. 2

(A) HeLa cells were transfected with SFTSV NSs-myc tagged or empty vector plasmid, and were subject to 2 h treatment with poly I:C (10 μg/ml) to mimic viral RNAinduced stimulation of the immune response. Indicated protein expression levels were analyzed by immunoblotting. Tubulin was used as a sample loading control. (B) HEK293T cells were co-transfected with SFTSV NSs-GFP tagged plasmid and RIG-IFLAG or TBK1-FLAG encoding plasmids. Co-localization of NSs was observed with RIG-I and TBK-1. Scale bars, 20 μm.