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. 2020 Dec 31;31(2):226–232. doi: 10.4014/jmb.2008.08048

Fig. 3. SFTSV NSs interfere with TBK1-induced IRF3 activation by inhibiting TBK1-IRF3 interaction.

Fig. 3

(A) HEK293T cells were transfected with plasmids encoding myc-tagged empty vector (EV) or SFTSV NSs and FLAG-tagged RIGI or TBK1. After 24 h post-transfection, cells were harvested and cell lysates were prepared for co-immunoprecipitation (IP) assay with rabbit anti-myc tag antibody. IP and whole cell lysates (WCL) were subjected to SDS-PAGE and immunoblot analysis with the indicated antibodies. The experiments were repeated three times. (B) HEK293T cells were transfected with plasmids encoding IRF3-GFP, TBK1-FLAG, and NSs-myc. Cells were harvested, lysed and immunoprecipitated with anti-FLAG tag antibody followed by SDS-PAGE and immunoblot analysis.