Table 2.

Design of parameter optimization in LAMP system.

Reaction conditions/unit	Parameters （increasing order）
Bst DNA polymerase/U	0, 1.6, 3.2, 4.8, 6.4, 8
Concentration ratio of inner primer to outer primer	4:1, 6:1, 8:1, 10:1, 12:1
Concentration of dNTP/mmol/l	0.64, 0.8, 0.96, 1.12, 1.28, 1.44, 1.6, 1.76
Concentration of Mg2+/mmol/l	1.2, 2.4, 3.6, 4.8, 6.0, 7.2
Reaction temperature/°C	57, 59, 61, 63, 65, 67
Reaction time/min	20, 30, 40, 50, 60, 70