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. 2021 Sep 4;31(12):1722–1731. doi: 10.4014/jmb.2106.06083

Fig. 2. Schematic diagram of the DNA fragment deletion process.

Fig. 2

Comparison of the the classical (A) and the SaindC-based (B) deletion process in Streptomyces. X, resistance gene cassette for antibiotic X; up, the upstream homologous arm of the target DNA fragment; and down, the downstream homologous arm of the target DNA fragment. (C) The flow of SaindC-based deletion process. Apr+, plates with apramycin added; Apr-, plates without apramycin added; original color colonies are indicated with red arrows.