Table 1.
Bacterial strains and vectors used in this work.
| Strains or vectors | Description | Reference |
|---|---|---|
| Strains | ||
| S. albidoflavus J1074 | Type strain of Streptomyces | [13] |
| S. chattanoogensis L10 | Industrial natamycin producing strain | CGMCC 2644 |
| S. chattanoogensis L10/ pINT01 | L10 carrying vector pINT01, apr | This study |
| S. chattanoogensis L10/ pSOK804 | L10 carrying vector pSOK804, apr | This study |
| S. chattanoogensis L10-Δazo | L10 with disruption of azoxymycin BGC | This study |
| S. albus ZD11 | A derivative obtained with streak plate method from an industrial salinomycin-producing strain | CGMCC 4.7658 |
| S. albus ZD11-Δsal | ZD11 with disruption of salinomycin BGC | This study |
| S. albus ZD11-Δ200k | ZD11 with disruption of 200 kb non-essential chromosomal region deleted | This study |
| S. coeruleorubidus | Daunorubicin producing strain purchased from CICC | CICC 11043 |
| S. coeruleorubidus -Δdnr | Streptomyces coeruleorubidus with disruption of daunorubicin BGC | This study |
| E.coli TG1 | Host strain for DNA clone | Stratagene |
| E.coli ET12567 (pUZ8002) | dam-dcm- strain containing helper plasmid pUZ8002 | [30] |
| Vectors | ||
| pKC1139S-kasOp | Dereived from pKC1139 containg kasOp*, apr | Unpublished |
| pSOK804 | Streptomyces / E. coli shuttle vector, apr | [31] |
| pINT01 | Derived from pSOK804 containing kasOp*-SaindC cassette, apr | This study |
| pSET152 | Streptomyces / E. coli shuttle vector, apr | [27] |
| pSUC01 | Derived from pSET152 containing kasOp*-SaindC cassette, apr | This study |
| pSUC02 | Derived from pSUC01 for the deletion of azoxymycin BGC, apr | This study |
| pSUC03 | Derived from pSUC01 for the deletion of daunorubicin BGC, apr | This study |
| pSUC04 | Derived from pSUC01 for the deletion of salinomycin BGC, apr | This study |
| pSUC05 | Derived from pSUC01 for the deletion of the 200 kb non-essential chromosomal region, apr | This study |
apr, apramycin resistance