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. 2021 Jun 14;31(8):1079–1087. doi: 10.4014/jmb.2105.05004

Fig. 4. Effects of GA treatment on the adipogenesis-related protein expression in 3T3-L1 cells.

Fig. 4

Western blotting was performed to analyze the expression of adipogenic proteins. After 8 days of inducing differentiation with or without GA treatment (300 and 600 μM), the cells were harvested and the proteins were extracted for analysis. (A) Protein expression levels of adiponectin, SREBP1, and FAS. (B) Protein expression and phosphorylation levels of PI3K pathway proteins (PI3K 110α, mTOR, and Akt). (C) Protein expression levels of β-catenin, GSK3β, and their phosphorylated forms. GAPDH is used as a loading control. GA, gentisic acid; MDI, mixture of IBMX, DEX, and insulin; SREBP1, sterol regulatory element-binding protein 1; FAS, fatty acid synthase; PI3K, phosphatidylinositol 3-kinase; mTOR, mammalian target of rapamycin; Akt, protein kinase B; GSK3β, glycogen synthase kinase 3β; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.