Fig. 4. The effect of L. sakei WIKIM31 administration on gut barrier function and inflammation response.

(A, B) Gene expression of pro-inflammatory cytokines was determined by real-time RT-PCR. (C) After isolated SVF were treated with LPS for 24 h, the levels of IL-6, TNF-α, and MCP-1 in the supernatant were detected using the Cytometric Bead Array kit. (D) Gene expression of tight junction proteins was determined by real-time RT-PCR. Data are presented as the mean ± SE. *p < 0.05 vs. HFD.