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. 2021 May 19;31(6):794–802. doi: 10.4014/jmb.2103.03057

Fig. 4. Silencing nuclear factor erythroid 2-related factor 2 (siNrf2) reversed the effect of cardamonin on interleukin (IL)-1β-induced expression of inflammatory factors and activation of nucleotide binding oligomerization domain-like receptor 3 (NLRP3 ) inflammasome in chondrocytes.

Fig. 4

(A) IL-6, IL-8 and tumor necrosis factor (TNF)-α mRNA expression levels were assessed by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) after treatment of IL-1β and cardamonin as well as transfection of siNrf2. (B and C) Representative images of NAD(P)H quinone dehydrogenase 1 (NQO-1), NLRP3, Caspase 1 and ASC protein bands (B) and NQO-1, NLRP3, Caspase 1 and ASC protein expression levels (C) were evaluated through western blot after treatment of IL-1β and cardamonin as well as transfection of siNrf2. β-Actin was used as a loading control. ***p < 0.001 vs. Control group; ^^^p < 0.001 vs. IL-1β group; ###p < 0.001 vs. IL-1β+Cardamonin+silencing negative control (siNC) group; ##p < 0.01 vs. IL-1β+Cardamonin+siNC group; #p < 0.05 vs. IL-1β+Cardamonin+siNC group. All experiments were repeated independently at least three times. Data were performed as the means ± SD.