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. 2021 Sep 15;31(12):1615–1623. doi: 10.4014/jmb.2109.09012

Fig. 1. Effects of PPT on Cell Proliferation and Apoptosis in HCT116 Cells.

Fig. 1

(A) Chemical structure of picropodophyllotoxin. (B) HCT116 cells were treated with various concentrations of PPT for 24 and 48 h. Cell viability was detected by the MTT assay. (C-E) HCT116 cells were plated in soft agar containing DMSO (control) and PPT (0.1, 0.2, and 0.3 μM) for 7 days. Colony number and size were analyzed and expressed relative to the DMSO-treated control value. (F and G) HCT116 cells were incubated with the indicated concentrations of PPT for 48 h and analyzed by the Muse cell Analyzer using the Muse Annexin V & Dead Cell Kit. Live cells (annexin-V negative/7-AAD negative) are on the bottom left side, early apoptotic cells (annexin-V positive/7-AAD negative) on the bottom right side, late apoptotic cells (annexin-V positive/7-AAD positive) on the top right side and necrotic cells (annexin-V negative/7-AAD positive) on the top left side. The data are expressed as the mean ± SD (n = 3). *p < 0.05, **p < 0.01, and ***p < 0.001 compared to the control group.