Table 1.
Genes | primer F (5'⇒3') | Primer R (5‘⇒3’) | Annealing temp | Cycles | Amplified DNA size (bp) | References |
---|---|---|---|---|---|---|
cagA5’region | GATAACAGGCAAGCTTTTGATG | CTGCAAAAGATTGTTTGGCAGA | 55°C | 35 | 349 | [16] |
cagA EPIYA FR | ACCCTAGTCGGTAATGGG | GCAATTTTGTTAATCCGGTC | 48°C | 30 | 293~299 | [72] |
cagA EPIYA JSR | GCAATTTTGTTAATCCGGTC | GCTTTAGCTTCTGAYACYGC | 48°C | 30 | 222 | [72] |
vacA s1/s2 | ATGGAAATACAACAAACACAC | CTGCTTGAATGCGCCAAAC | 52°C | 35 | 259/286 | [73] |
vacA s1a s1a | TCTYGCTTTAGTAGGAGC | CTGCTTGAATGCGCCAAAC | 52°C | 35 | 212 | [16] |
vacA s1b ss3 | AGCGCCATACCGCAAGAG | CTGCTTGAATGCGCCAAAC | 55°C | 35 | 187 | [73] |
vacA s1c s1c | CTYCTTTAGTRGGGYTA | CTGCTTGAATGCGCCAAAC | 55°C | 35 | 213 | [16] |
vacA m1/m2 | CAATCTGTCCAATCAAGCGAG | GCGTCTAAATAATTCCAAGG | 52°C | 35 | 570/645 | [16] |
vacA i1 | TTAATTTAACGCTGTTTGAAG | GTTGGGATTGGGGGAATGCCG | 53°C | 35 | 426 | [74] |
vacA i2 | GATCAACGCTCTGATTTGA | GTTGGGATTGGGGGAATGCCG | 53°C | 35 | 432 | [74] |
alpA | ACGCTTTCTCCCAATACC | AACACATTCCCCGCATTC | 65°C | 40 | 304 | [75] |
alpB | TCGCCGGGACTTTGGGCAAC | TGCGCTAAAGCGGCGTCCAA | 55°C | 35 | 505 | Designed |
oipA | CAAGCGCTTAACAGATAGGC | AAGGCGTTTTCTGCTGAAGC | 55°C | 35 | 450 | [76] |
babA2 | AATCCAAAAAGGAGAAAAAACATGAAA | ATAGTTGTCTGAAAGATC | 50°C | 38 | 180 | [26] |
hopZ | GCCTGATATGGGTGGCATGGG | ATTTGATAGCCCGCGCTGAT | 50°C | 35 | 493 | [77] |
sabA | GAGCTATTGACCAGCTCAATG | TAGTTTGGATTCGTTCTCATTA | 50°C | 35 | 447 | [78] |
iceA1 | TATTTCTGGAACTTGCGCAACCTGAT | GGCCTACAACCGCATGGATAT | 55°C | 30 | 642 | [79] |
iceA2 | CGGCTGTAGGCACTAAAGCTA | TCAATCCTATGTGAAACAATGATCGTT | 55°C | 30 | 662 | [79] |
iceA1Δ94 | GGTGAGTCGTTGGGTAAGCGTTACAGAATT | CACAACCATCATATTCAGCCTCCCCCTCATA | 55°C | 30 | 520 | [79] |
hrgA | TCTCGTGAAAGAGAATTTCC | TAAGTGTGGGTATATCAATC | 55°C | 30 | 682 | [80] |
hpyIIIR | CTCATTGCTGTGAGGGAT | TCTTGATAGGATCTTGCG | 55°C | 30 | 443 | [80] |
hpyIIIM | CTCATTGCTGTGAGGGAT | TCTTGATAGGATCTTGCG | 55°C | 30 | 562 | [80] |
dupA jhp0917 | TGGTTTCTACTGACAGAGCGC | AACACGCTGACAGGACAATCTCCC | 57°C | 30 | 307 | [22] |
dupA jhp0918 | CCTATATCGCTAACGCGCGCTC | AAGCTGAAGCGTTTGTAACG | 57°C | 30 | 276 | [22] |