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. 2021 Feb 8;31(3):368–379. doi: 10.4014/jmb.2101.01023

Table 1.

Sequences of oligonucleotide primers and PCR conditions for the genotyping of H. pylori isolates.

Genes primer F (5'⇒3') Primer R (5‘⇒3’) Annealing temp Cycles Amplified DNA size (bp) References
cagA5’region GATAACAGGCAAGCTTTTGATG CTGCAAAAGATTGTTTGGCAGA 55°C 35 349 [16]
cagA EPIYA FR ACCCTAGTCGGTAATGGG GCAATTTTGTTAATCCGGTC 48°C 30 293~299 [72]
cagA EPIYA JSR GCAATTTTGTTAATCCGGTC GCTTTAGCTTCTGAYACYGC 48°C 30 222 [72]
vacA s1/s2 ATGGAAATACAACAAACACAC CTGCTTGAATGCGCCAAAC 52°C 35 259/286 [73]
vacA s1a s1a TCTYGCTTTAGTAGGAGC CTGCTTGAATGCGCCAAAC 52°C 35 212 [16]
vacA s1b ss3 AGCGCCATACCGCAAGAG CTGCTTGAATGCGCCAAAC 55°C 35 187 [73]
vacA s1c s1c CTYCTTTAGTRGGGYTA CTGCTTGAATGCGCCAAAC 55°C 35 213 [16]
vacA m1/m2 CAATCTGTCCAATCAAGCGAG GCGTCTAAATAATTCCAAGG 52°C 35 570/645 [16]
vacA i1 TTAATTTAACGCTGTTTGAAG GTTGGGATTGGGGGAATGCCG 53°C 35 426 [74]
vacA i2 GATCAACGCTCTGATTTGA GTTGGGATTGGGGGAATGCCG 53°C 35 432 [74]
alpA ACGCTTTCTCCCAATACC AACACATTCCCCGCATTC 65°C 40 304 [75]
alpB TCGCCGGGACTTTGGGCAAC TGCGCTAAAGCGGCGTCCAA 55°C 35 505 Designed
oipA CAAGCGCTTAACAGATAGGC AAGGCGTTTTCTGCTGAAGC 55°C 35 450 [76]
babA2 AATCCAAAAAGGAGAAAAAACATGAAA ATAGTTGTCTGAAAGATC 50°C 38 180 [26]
hopZ GCCTGATATGGGTGGCATGGG ATTTGATAGCCCGCGCTGAT 50°C 35 493 [77]
sabA GAGCTATTGACCAGCTCAATG TAGTTTGGATTCGTTCTCATTA 50°C 35 447 [78]
iceA1 TATTTCTGGAACTTGCGCAACCTGAT GGCCTACAACCGCATGGATAT 55°C 30 642 [79]
iceA2 CGGCTGTAGGCACTAAAGCTA TCAATCCTATGTGAAACAATGATCGTT 55°C 30 662 [79]
iceA1Δ94 GGTGAGTCGTTGGGTAAGCGTTACAGAATT CACAACCATCATATTCAGCCTCCCCCTCATA 55°C 30 520 [79]
hrgA TCTCGTGAAAGAGAATTTCC TAAGTGTGGGTATATCAATC 55°C 30 682 [80]
hpyIIIR CTCATTGCTGTGAGGGAT TCTTGATAGGATCTTGCG 55°C 30 443 [80]
hpyIIIM CTCATTGCTGTGAGGGAT TCTTGATAGGATCTTGCG 55°C 30 562 [80]
dupA jhp0917 TGGTTTCTACTGACAGAGCGC AACACGCTGACAGGACAATCTCCC 57°C 30 307 [22]
dupA jhp0918 CCTATATCGCTAACGCGCGCTC AAGCTGAAGCGTTTGTAACG 57°C 30 276 [22]