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. 2022 Nov 15;9:1005616. doi: 10.3389/fvets.2022.1005616

Table 6.

Parameters estimated by applying the sigmoid model to evaluate the antibacterial activity index for S. aureus at pH 7.4, 6.5, and 5.0 vs. erythromycin in the presence of EO.

EO-ERY ATCC 29213 Sensitive strains Resistant strains
pH 7.4 6.5 5.0 7.4 6.5 5.0 7.4 6.5 5.0
R 0.99 0.99 1.00 0.99 0.99 0.99 0.99 0.99 1.00
Emax (Log10 CFU/mL) 10.69 7.62 7.38 10.79 7.94 8.08 13.85 8.26 5.12
0.64 1.35 3.89 1.27 1.99 1.61 0.39 1.19 1.87
C50 (μg/mL) of ERY 0.03 0.08 0.84 0.04a 0.11a 0.53b 189.50c 33.59d 61.15e
E0 (Log10 CFU/mL) 6.11 5.01 4.48 6.87 4.59 4.34 5.34 4.97 1.89

Sigmoid model. E0, E index in the absence of antimicrobials; Emax, maximum reduction in bacterial count; C50, concentration of erythromycin (in the presence of EO) necessary to achieve 50% of the maximum antibacterial effect; ⋎, sigmoidicity coefficient. Wild strains were grouped by minimal inhibitory concentration (MIC) similarity. Different lowercase letters between the pHs mean statistical differences (p < 0.05). Values without significant differences have the similar letter (a, b, c, d, e).