Fig. 6. A-F the indicated gram-positive bacteria were cultured with various treatments; negative control (PL-), AE alone (32 μg/ml) (P+L-), light alone (72 J/cm²) (P-L+), AE and light (P+L+).

The supernatants were then used to assess the hemolytic activity. The observed hemolytic activity of culture bacteria supernatants co-cultured with various treatments were A: S. aureus (ATCC 29213) 89.67% (P-L-) 83.33 % (P-L+) 68.33 % (P+L-) and 24% (P+L+) B: MRSA; 89% (PL-) 81.67% (P-L+) 65.67% (P+L-) and 21.67% (P+L+) C: E. faecalis (ATCC 29212) 92.33% (P-L-) , 81.67% (P-L+) 65.33% (P+L-) and 17 % (P+L+) D: VRE (ATCC 51299): 93% (P-L-) 81% (P-L+) 61.33% (P+L-) and 13.3% (P+L+) E: S. pneumoniae (ATCC 49619); 93.67% (P-L-) 83.33% (P-L+) 63.67% (P+L-) and 23.66% (P+L+). F: MDR-S pneumoniae: 93.33% (P-L-) 84.33% (P-L+) 62% (P+L-) and 21% (P+L+). ***Shows p < 0.0001, compared with the untreated (P-L-) group; two-tailed t-test.