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. 2021 Feb 12;31(3):483–491. doi: 10.4014/jmb.2012.12036

Fig. 4. Effects of pH and temperature on the activity of the recombinant Lip29 and Est29.

Fig. 4

(A and D) For the determination of optimal pH, activity was measured in sodium acetate buffer (pH 4.0-6.0), sodium phosphate buffer (pH 6.0-8.0), Tris-HCl buffer (pH 8.0-9.0), and bicarbonate-carbonate buffer (pH 9.0- 10.5). (B and E) For the determination of optimal temperature, activity was measured at pH 9.5 (Lip29) and pH 6.0 (Est29). (C and F) For the determination of thermostability, the enzyme was incubated in 50 mM bicarbonate-carbonate buffer, pH 9.5 (Lip29) and 50 mM sodium phosphate buffer, pH 6.0 (Est29) at 50°C (closed circles), 60°C (open rectangulars), 65°C (closed rectangulars), 70°C (open circles), 90°C (closed reverse triangles) for 30 min intervals.