Table 1.
Effect of different cations and detergents on Lip29 activity.
| Compounds | Specific activity (U/mg) | Relative activity (%) |
|---|---|---|
| None | 10.7 ± 0.21 | 100 |
| MgSO4 | 10.4 ± 0.62 | 98 |
| MgCl2 | 10.1 ± 0.27 | 95 |
| KCl | 10.5 ± 0.29 | 98 |
| NaCl | 10.2 ± 0.19 | 97 |
| MnCl2 | 9.6 ± 0.45 | 90 |
| CaCl2 | 10.1 ± 0.12 | 94 |
| CuCl2 | 1.1 ± 0.15 | 10 |
| FeCl3 | 9.1 ± 0.05 | 85 |
| C°Cl2 | 10.4 ± 0.50 | 97 |
| ZnCl2 | 8.0 ± 0.46 | 82 |
| SDS | 3.0 ± 0.22 | 24 |
| DMSO | 12.2 ± 0.10 | 99 |
| Triton X-100 | 11.2 ± 0.98 | 91 |
| Tween 80 | 5.9 ± 0.36 | 48 |
| Tween 20 | 10.8 ± 0.12 | 88 |
Activity is expressed as mean ± standard deviation from three independent experiments.
The cation and detergent concentration used in this study was 1 mM and 1%, respectively.