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. 2021 Sep 25;31(11):1559–1567. doi: 10.4014/jmb.2109.09002

Fig. 6. Apoptotic effects of ursolic acid on RPMI-8226 cell growth.

Fig. 6

(A) RPMI-8226 cells were incubated with the DMSO (vehicle) or ursolic acid (20 and 40 μM) for 48 h. After incubation, cells were harvested and stained with propidium iodide (PI) and analyzed using a cytometer. The x-axis indicates the PI fluorescence intensity that correlated with the DNA content. (B) RPMI-8226 cells were incubated with the DMSO (vehicle) or ursolic acid (20 and 40 μM) for 48 h. After incubation, cells were harvested and stained with annexin V-FITC and PI and analyzed using a cytometer. The x-axis indicates the annexin V-FITC intensity, while the y-axis indicates the PI fluorescence. (C) Western blot with anti-caspase-3, anti-poly (ADP-ribose) polymerase (PARP), and anti-actin (loading control) antibodies on whole-cell extracts. The results are representative of three independent experiments. (D) Caspase-3/7 activity determined after RPMI8226 cell incubation with either DMSO (vehicle) or ursolic acid (20 and 40 μM) for 48 h. The results represent the mean ± SD of three independent experiments. *p < 0.05 compared with the DMSO control group.