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Immunoblot analysis of mycobacterial cell lysates. Equivalent amounts of cellular proteins (10 μg/lane) were separated on a 13% polyacrylamide gel, transferred to nitrocellulose filters, and probed with a monoclonal antibody. Panels: A, pANCA Fab 5-3; B, anti-tetanus toxoid Fab P313. The values to the left of each panel are molecular masses in kilodaltons.
