FIG. 2.

Direct protein sequencing identifies the reactive protein as a mycobacterial homologue of histone H1. The N-terminal protein sequence from the biochemically isolated immunoreactive mycobacterial protein (Peptide) is aligned with two homologue mycobacterial proteins identified in the NIH nonredundant sequence database, Z83018 from M. tuberculosis (M. Tb.) and Z99263 from M. leprae, and the H1.5 isoform of human histone H1. Strain H37Rv (shown) and strain Erdman differed at aa 208 by a single missense polymorphism resulting in alanine and threonine, respectively (bold). Dashes are spaces for alignment of sequences defined by the CLUSTAL program.