FIG. 3.

Fab 5-3 binding to HupB and histone H1 GST fusion proteins. Equivalent numbers of bacteria expressing recombinant GST fusions with histone H1 (aa 69 to 171), HupB, or GST alone were subjected to SDS–13% PAGE and electrotransferred to nitrocellulose membranes. Membranes were analyzed by silver staining for protein composition (left panel), immunoblotted with anti-GST to detect GST fusion protein expression (middle panel), or immunoblotted with Fab 5-3 to detect expression of the Fab 5-3 pANCA epitope (right panel). Arrows to right of each panel indicate sizes of full-length recombinant products for GST, GST-H1(69-171), and GST-HupB (29, 41, and 54 kDa, respectively). Smaller products detected by immunoblotting are proteolytic fragments of the full-length products. The values to the left are molecular masses in kilodaltons.