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. 2022 Nov 15;12:1023072. doi: 10.3389/fonc.2022.1023072

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Copyright © 2022 Parisi, Mockenhaupt, Rihs, Mansour, Katsaros and Degen

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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IRF6 and GRHL3 expression in healthy human adult tissues and cells (A) QPCR analysis of IRF6 and GRHL3 in a panel of healthy human adult tissues. Color code refers to tissue groups introduced in Supplementary Figure 1A . (B) IHC for IRF6 as well as a representative H&E staining of a set of normal human tissues. Note that IRF6 exhibits nuclear as well as cytoplasmic localization. Scale Bar: 50 µm. (C) QPCR analysis of IRF6 and GRHL3 in a panel of healthy human cell strains/lines. not detectable (cT > 32) (D) Immunoblot for the proteins IRF6 and E-Cadherin in a set of human cell strains/lines. Note the typical IRF6 double-band indicative of its phosphorylated and non-phosphorylated form, respectively. Full-length blots are shown in Supplementary Figure 6 kDa, kilo Daltons.