Figure 10.

Overexpression of SRSF1 affects HIV-1 infectivity and viral particle production. HEK293T cells were co-transfected with a plasmid coding for the proviral clone NL4-3 PI952 (pNL4-3 PI952) (371) and a plasmid coding for FLAG-tagged SRSF1 (pcDNA-FLAG-SF2) or an empty vector [pcDNA3.1(+)] as mock control. (A, B) Seventy-two hours post-transfection, the cell culture supernatant was used to determine viral infectious titers using TZM-bl reporter cells. (A) X-Gal staining of TZM-bl cells incubated with the cellular supernatant. (B) Measurement of luciferase activity. (C) Viral RNA extracted from the supernatant was subjected to RT-qPCR to quantify absolute expression levels of exon 7-containing transcripts (total viral mRNA). (D) p24-CA ELISA was performed to determine viral particle production. Statistical significance was calculated using unpaired two-tailed t-tests (****p < 0.0001 and ns, not significant). Mean ( ± SEM) of n = 4 biological replicates is depicted for (B–D).