Figure 3.

SRSF1 expression upon stimulation with IFNα subtypes. Differentiated THP-1 cells were treated with the indicated IFN subtype (10 ng/ml). Twenty-four hours post-treatment, cells were harvested, and RNA was isolated and subjected to RT-qPCR for measurement of relative (A) ISG15 and (B) SRSF1 mRNA expression levels. Statistical significance was analyzed performing one-way ANOVA with Dunnett post-hoc test (*p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001). Mean ( ± SEM) of n = 3 biological replicates is depicted. (C–E) Differentiated THP-1 cells were treated with 1 µM Ruxolitinib or DMSO as mock control1 h before infection using NL4-3 AD8 (1 MOI). Sixteen hours post-infection, cells were washed with PBS and treated with media containing PBS or IFNα14, and either Ruxolitinib or DMSO. At the indicated time points, wells were rinsed with PBS, and cells were subjected to RNA isolation and RT-qPCR analysis to monitor mRNA expression levels of (C) ISG15, (D) IFITM1, and (E) SRSF1. Groups were compared with two-way repeated-measures ANOVA with Tukey’s post-hoc test. ns is not significant.