Figure 6.

IFNα14-mediated changes in newly transcribed SRSF1 mRNA. (A, B) THP-1 macrophages were stimulated with IFNα14 (10 ng/ml). 4-Thiouridine (4sU) was added 30 min before harvesting the cells at the indicated time points in order to label newly synthesized RNA. After separation and isolation of the freshly synthesized RNA, RT-qPCR was performed to measure relative mRNA expression levels of (A) ISG15 and (B) SRSF1. GAPDH was used as a house-keeping gene for normalization. Statistical significance compared to untreated control was determined using unpaired two-sided Welch’s t-test. Asterisks indicated p-values as *p < 0.05 and ns, not significant. Mean ( ± SEM) of n = 3 biological replicates is shown (except for 4 h, where n = 2).