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. 2022 Sep 21;190(4):2775–2796. doi: 10.1093/plphys/kiac441

Figure 10.

Figure 10

SlARF2 promoters are a direct target of SlERF.D7. A, SlERF.D7 binding to the promoters of SlARF2 harboring the ERE element. The WT probe containing the ERE motif was digoxigenin-labeled. Competition for SlERF.D7 binding was performed with 8× and 15× cold probes. The symbols − and + represent the absence or presence of the probes and 6× Histidine-tagged SlERF.D7 protein. B, Phenotypes of SlARF2A, SlARF2B, and SlARF2AB silenced fruits in SlERF.D7 OE background. Un-infiltrated and vector-only (pTRV) fruits were used as the control. Photographs were taken at MG, 3-day post Br (Br+3), and 7-day post Br (Br+7) stages. C, The silencing efficiency of the SlARF2 orthologs in SlARF2A, SlARF2B, and SlARF2AB infiltrated fruits. The relative mRNA levels of both the genes were normalized using GAPDH and Actin gene as an internal control. Error bars represent ±sd of three biological replicates. Asterisks indicate the statistical significance using Student’s t test: *, 0.01 < P-value < 0.05; **, 0.001 < P-value < 0.01.