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. 2022 Sep 21;190(4):2775–2796. doi: 10.1093/plphys/kiac441

Figure 4.

Figure 4

Pigment accumulation assessment in SlERF.D7 OE and RNAi fruits. A and B, Estimation of (A) lycopene and (B) β-carotene in WT and SlERF.D7 OE and RNAi lines at different stages of fruit ripening. C, RT-qPCR analysis of carotenoid biosynthetic pathway genes in WT and SlERF.D7 OE and RNAi tomato lines at MG, Br, 3-day after Br (Br+3), 7-day after Br (Br+7), and 10-day after Br (Br+10) with Actin gene as an internal control. Error bars are means ±sd of three biological replicates. Asterisks indicate the statistical significance using Student’s t test: *, 0.01 < P-value < 0.05; **, 0.001 < P-value < 0.01. β-LCY1, β-LCY2, CYC-β lycopene b-cyclases; PSY1 phytoene synthase; PDS phytoene desaturase; and ZDS, carotenoid desaturase.