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. 2022 Sep 21;190(4):2775–2796. doi: 10.1093/plphys/kiac441

Figure 8.

Figure 8

Altered auxin levels and ARF2 orthologs transcription in SlERF.D7 transgenic fruits. A, Determination of IAA levels during fruit ripening in WT, SlERF.D7 OE, and RNAi line fruits at MG, Br, and 7-day post Br (Br + 10) stages. Error bars represent ±sd of three biological replicates. Asterisks indicate the statistical significance using Student’s t test: *, 0.01 < P-value < 0.05; **, 0.001 < P-value < 0.01. B, Expression profiling of SlARF2 orthologs, SlARF2A and SlARF2B in transgenic fruits of SlERF.D7 OE and RNAi lines during different fruit maturation stages, MG, Br, 3-day after Br (Br+3), 7-day after Br (Br+7), and 10-day after Br (Br+10). The relative mRNA levels of both the genes were normalized using Actin and GAPDH genes as an internal control. Error bars represent ±sd of three biological replicates. Asterisks indicate the statistical significance using Student’s t test: *, 0.01 < P-value < 0.05; **, 0.001 < P-value < 0.01. C, Analysis of putative ethylene and auxin-responsive cis-elements in the 2.5-kb promoter region of SlARF2A and SlARF2B genes. D, Y1H analysis of binding of SlERF.D7 protein to putative ethylene-responsive elements in the promoter regions of SlARF2A and SlARF2B genes displaying growth performance of transformants on SD/−Leu−/Trp/−His medium containing 50 mM 3-AT. Binding of RIN to the promoter of SlACS2 gene-: positive control, binding of SlERF.D7 to the promoter of SlARF3 gene-: negative control.