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. 2022 Sep 29;190(4):2812–2827. doi: 10.1093/plphys/kiac456

Figure 5.

Figure 5

CycC1;1 represses ABI5 expression during seed germination. A, The expression of ABI5 in germinating seeds of the WT, cycc1;1, and CycC1;1-OE lines. Seeds were treated with MS medium or MS medium containing 10 μM ABA for 24 h. Error bars indicate mean ± sd (n = 3). B and C, GUS staining of germinating seeds (B) and seedlings (C) of ABI5::GUS transgenic line in the WT, cycc1;1 and CycC1;1-OE background plants. One-day-old and 7-day-old seedlings grown on MS medium were transferred to MS medium containing 10 μM ABA for 24 h, and then subjected to GUS staining. Bar, 0.2 cm. D, ABI5 protein abundance in the seeds of WT, cycc1;1 and CycC1;1-OE lines. Seeds were treated with MS medium without or with 10 μM ABA for 24 h. The anti-ABI5 antibody was used in the immune analysis. E and F, Dual-LUC reporter gene assay to examine the effects of ABI5 and CycC1;1 on EM1 expression. The intact ABI5 genomic DNA containing its promoter, coding region, and terminator was cloned into a binary vector to transiently express ABI5 protein in N. benthamiana leaves. The activity of EM1::LUC alone was set to 1. Error bars indicate mean ± sd (n = 3). Different letters indicate significant differences as determined using one-way ANOVA followed by Tukey’s multiple comparison test (P < 0.05).