Figure 3.

Phenotypes of Col-0 wild-type, bicat3-1, and bicat3-2 under Mn²⁺ deficiency conditions. A, Growth phenotypes of 5-week-old plants cultivated in hydroponics with 3.5 µM Mn²⁺ (control) or 0.05 µM Mn²⁺ (-Mn). Images were digitally extracted for comparison. Scale bar represents 1 cm and applies to all images. B, Shoot and root DW of plants grown as in (A). C and D, Mn²⁺ and Ca²⁺ concentrations in shoots and roots of plants grown as in (A). Data indicate means + se of five biological replicates. Data were analyzed by two-tailed Student’s t test to identify significant differences between wild-type and mutant (^*P < 0.05; ^**P < 0.01; ^***P < 0.001). The experiment was repeated twice with similar results.