Figure 2.

Proposed mechanism for the 6-HDNO-EneIRED cascade. (a) Kinetic profile from in situ ¹⁹F NMR reaction monitoring of THP-6b. ^aReactions run at 1 mmol. (b) Enamine 10e is used to probe the role of this species as an intermediate in the cascade. (c) Proposed catalytic sequence for the AmOxEneIRED biocatalytic cascade. ^bTwo equivalents of NAD(P)H consumed to form piperidine. (d) Structure of the dimer of EneIRED-07 in the ribbon format with subunits shown in green and blue. NADP⁺ can be seen bound at the two active sites. (e) Active site of EneIRED-07 with the (S)-enantiomer of iminium ion intermediate 3f modeled into the active site.