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. 2022 Nov 14;12(18):7884–7902. doi: 10.7150/thno.73218

Figure 4.

Figure 4

Comparing digital cytometry results for two-component treatment with aCD40 + aPD-1 or ablation + aPD-1 in the MT4 pancreatic and NDL breast cancer models. aCD40 + aPD-1 increases leukocytes and activated dendritic cell numbers. Digital cytometry was applied to bulk RNA sequencing data acquired in the MT4 and NDL models under the protocol in Figure 3A. A-C) MT4 model. D-F) NDL model. A, D) CIBERSORTx absolute score. B, E) Fold change from the no treatment control (NTC) cohort, plotted between ablation + aPD-1 in the directly-ablated tumor (A-aPD-1-T) and aCD40 + aPD-1 (aCD40-aPD-1). C, F) Fold change from the NTC cohort, plotted between ablation + aPD-1 in the distant tumor (A-aPD-1-C) and aCD40 + aPD-1 (aCD40-aPD-1). Note that log ratios are based on CIBERSORTx absolute scores. RNAseq experiments were performed with n = 4 replicates with a negative binomial test and Bonferroni correction for p values. Expression of genes in the grey region of E-F was zero in the NTC. Abbreviations: Mast cells (MCs), Polymorphonuclear leukocytes (PMN).