Figure 7.

Combination of ablation with CP4 in the MT4 tumor model generates a systemic anti-tumor effect. Tumor growth from the protocols shown in Figure 6A (n=4 for treatments and n=3 for the no treatment control (NTC) cohort). A) Survival for NTC, Ablation alone, Ablation + aPD-1, Ablation + aCTLA-4, Ablation + aCD40, CP4 alone, Ablation + CP4 cohorts. B-G) Tumor growth for B) NTC, C) CP4, D) Ablation in the treated tumor, E) Ablation in the distant tumor, F-G) Ablation + CP4 in the treated (F) and distant (G) tumor. Tumor volume plots are provided in Figure S16. H) Cox-hazard analysis comparing cells to survival outlined the importance of activated dendritic cells. I) Pearson correlation analysis indicated high correlations between 1) survival and dendritic cell (DC) activation, 2) NK cells and CD4⁺ T cells and 3) plasma cells (PCs) and dendritic cell activation.