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. 2022 Nov 7;12(18):7668–7680. doi: 10.7150/thno.75965

Figure 4.

Figure 4

Aβ-specific Tregs suppress neuroinflammation in the microglia of 3xTg-AD mice. (A) Brain sections from 3xTg-AD mice were stained for Iba1 to detect activated microglia. The boxed areas indicate magnified areas. The scale bar represents 20 μm. (B) The levels of Iba1 and (C) GFAP in the hippocampus of 3xTg-AD mice were measured by western blotting. (D) Adult microglia were isolated using CD11b microbeads from the brain and mRNA was extracted for Quantseq 3`mRNA-seq. (E) RNAseq scatter plot showing M1-associated genes. (F) Heatmap of 33 microglia-associated genes differentially regulated in the Non-Tg versus 3xTg-AD mice. (G) The mRNA expression of the proinflammatory cytokines IL-1β, TNF-α, and IL-23 and (H) the anti-inflammatory cytokines Arg-1 and YM1 in the hippocampus of 3xTg-AD mice was also measured by RT-PCR. (I) The expression of NOS2 was detected in the hippocampus of 3xTg-AD mice of different ages (scale bar represents 100 μm), (J) and the staining intensity was measured. Data are presented as the mean ± SEM. n = 5; * P < 0.05, ** P < 0.01, *** P < 0.001.