TABLE 1.
Plasmids used
| Plasmid | Vector | Relevant properties | Source or reference |
|---|---|---|---|
| pGEX-5X-3 | E. coli expression vector | Pharmacia Biotech | |
| pK184 | E. coli expression vector | 38 | |
| pET21d | E. coli expression vector | Novagen | |
| pFLAG-MAC | E. coli expression vector | Sigma | |
| pROEX-1 | E. coli expression vector | Life Technologies | |
| pEF-BOS | Mammalian expression vector | Nagata | |
| pEOSW30 | pK184 | 3,332-bp BanI-HoeII fragment carrying cnf2 | 38 |
| pSPEO1/2 | pK184 | 3,000-bp SmaI fragment carrying cnf1 | 37 |
| pGST-RhoA | pGEX-5X-3 | H. Maruta | |
| pGST-Rac1 | pGEX-5X-3 | H. Maruta | |
| pGST-Cdc42 | pGEX-5X-3 | A. Kikuchi | |
| pGST-P122RhoGAP | pGEX-2T | Y. Homma | |
| pFLAG-RhoA | Y. Horiguchi | ||
| pFLAG-RhoA63E | Y. Horiguchi | ||
| pFLAG-Rac1 | pFLAG-MAC | This study | |
| pFLAG-Cdc42 | pFLAG-MAC | This study | |
| pSPEO6/33 | pROEX-1 | 1,014-bp fragment of cnf2 (entire cnf2 structural gene) | This study |
| pSPEO3/3 | pROEX-1 | 497-bp fragment from nucleotides 1 to 497 of cnf2 (N-terminal region of cnf2) | This study |
| pEOFH3 | pROEX-1 | 872-bp fragment from nucleotides 442 to 1014 of cnf2 (C-terminal region of cnf2) | This study |
| pMH-RhoA | pEF-BOS | EcoRI fragment of pFLAG-RhoA | This study |
| pMH-RhoA63E | pEF-BOS | EcoRI fragment of pFLAG-RhoA63E | This study |
| pMH-Rac1 | pEF-BOS | EcoRI fragment of pFLAG-Rac1 | This study |
| pMH-Cdc42 | pEF-BOS | EcoRI fragment of pFLAG-Cdc42 | This study |