Table 1.
The sequences of genes for RT-qPCR.
| Gene | Primer | Sequence (5′-3′) | PCR products |
|---|---|---|---|
| 18S | Forward | TAGTTGGTGGAGCGATTTGTCT | 169bp |
| Reverse | CGGACATCTAAGGGCATCACA | ||
| csal1 | Forward | CCCAGGAAGCAAGGTGTA | 167bp |
| Reverse | CGTTGGCATGTCCGTATT | ||
| BMP5 | Forward | GTGAGCAAAAGCAAGCAT | 260bp |
| Reverse | ATCAAAGTAGAGCACGGAG | ||
| TACR2 | Forward | CAGATTTGCTGATGTCCG | 310bp |
| Reverse | CACTTTGTTGTCCCGTTG | ||
| AMH | Forward | GGAGGAGATGGGACTTGGAG | 261bp |
| Reverse | AAGGCACTTTCATAGCGGG | ||
| PLAG1 | Forward | ATGATGGGTCTGGGGAGGT | 311bp |
| Reverse | TCGTGGTAGGGTGGTGGTT | ||
| SLC7A11 | Forward | GAAAAAAACATACCCCTCG | 214bp |
| Reverse | CACCATTCATAGACCCAAA | ||
| MYOD1 | Forward | CACGGGAACCCACACGAGGA | 352bp |
| Reverse | AGCGAGGGCTGGAGGCATCT | ||
| BOP1 | Forward | CAGAGGAAGATGAGGGTGAACG | 173bp |
| Reverse | TCGGAGCCTGATGCCAAC | ||
| SIPA1 | Forward | GCCTCGGCTACATCCCCAAT | 168bp |
| Reverse | GACCCCACCGCTCATCTTCA | ||
| NOTCH1 | Forward | CTGGGGACACCACCTACGA | 195bp |
| Reverse | GCTGGCACTCATCCACATC | ||
| BCL2L1 | Forward | CTTTCAGCGACCTCACCTCC | 192bp |
| Reverse | ACACAATGCGTCCCACCA | ||
| SOX9 | Forward | AATACCTGCCCCCCAACG | 382bp |
| Reverse | TACTGCGAGCGGGTGATG | ||
| ADGRA2 | Forward | TACACCAACGACATCACCCG | 146bp |
| Reverse | GCCACATAGAGGACATCAACCA | ||
| WNT5A | Forward | CTTCAACGCACCCACCAT | 151bp |
| Reverse | GCAGCACATCAGTTCACAGC | ||
| GATAD2B | Forward | GCTGGTGCTGCTGAAGAAG | 105bp |
| Reverse | GGAGATGGCTGGACGATAGA |