Table 1. Kinetic Parameters Associated with the Set of Aptananozymes I–V and Control Systems, and Dissociation Constants of the Respective Dopamine and Aptananozymes^a.

aptananozyme	Vmax (μM min–1)	KM (μM)	kcat (s–1)	Kd (μM)
IV	2.05 ± 0.11	113 ± 13	3.8 ± 1.0	0.76 ± 0.03
III	1.95 ± 0.22	207 ± 67	6.5 ± 0.8	0.95 ± 0.04
II	1.73 ± 0.26	247 ± 36	5.7 ± 0.8	1.20 ± 0.06
I	1.66 ± 0.13	305 ± 67	5.5 ± 0.9	1.65 ± 0.03
V	0.89 ± 0.31	310 ± 56	3.0 ± 0.5	0.93 ± 0.05
scrambled DBA/pA-AuNPs	0.43 ± 0.05	334 ± 89	1.4 ± 0.6	–
separated DBA/pA-AuNPsb	0.2 ± 0.06	–	0.7 ± 0.08	–

^aAll experiments were performed in a 5 mM MES buffer solution, pH 5.5, that included 5 mM MgCl₂, 100 mM NaCl, and 5 nM of the respective aptananozymes or control system and 5 mM H₂O₂. Error bars derived from N = 3 experiments.

^bThe separated pA-AuNPs/DBA system shows pseudo-first-order kinetics: k = 0.7 s^–1.