Table 2. Kinetic Parameters Associated with the Set of Aptananozymes I–V and Control Systems, and Accompanying Dissociation Constants of the Respective Dopamine and Aptananozymes^a.

aptananozymes	Vmax (μM min–1)	Km (μM)	kcat (s–1)
IV	0.50 ± 0.02	71 ± 11	1.7 ± 0.20
III	0.42 ± 0.02	72 ± 17	1.4 ± 0.15
II	0.38 ± 0.01	76 ± 12	1.3 ± 0.13
I	0.31 ± 0.02	77 ± 13	1.0 ± 0.08
V	0.25 ± 0.02	90 ± 20	0.8 ± 0.09
scrambled DBA/pA-AuNPs	0.12 ± 0.01	113 ± 32	0.4 ± 0.06
separated DBA/pA-AuNPsb	0.038 ± 0.06	388 ± 52	0.1 ± 0.01

^aAll experiments were performed in a 5 mM MES buffer solution, pH 5.5, that included 5 mM MgCl₂, 100 mM NaCl, and 5 nM of the respective aptananozymes or control system and 5 mM H₂O₂. Error bars derived from N = 3 experiments.

^bThe separated pA-AuNPs/DBA system shows pseudo-first-order kinetics: k = 0.1 s^–1.