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Control experiments to confirm localized free radical sensing. NLS-FNDs at the nucleus, FNDs in the cytosol, and MIT-FNDs at the mitochondria were exposed to diazoxide (50 μM) which triggers free radical generation at the mitochondria. The graphs are made based on six replicates of each variant, and error bars represent averages ± standard deviation (****P ≤ 0.0001).
