Table 4.
PCR Protocols for RNA and DNA virus detection
| RNA Virus | PCR Protocol | References |
|---|---|---|
| Flavivirus | cDNA synthesis followed by PCR end point | [46], Additional file 1 |
| Coronavirus | cDNA synthesis followed by PCR end point | [47], Additional file 1 |
| EMCV | cDNA synthesis followed by PCR end point | [48], Additional file 1 |
| EMCV | Real time RT-PCR | Kindly provided by dr E.A. Foglia, IZS Lombardia ed Emilia Romagna, Italy |
| Morbillivirus | cDNA synthesis followed by PCR end point | [49], Additional file 1 |
| WNV Lineage 1 and 2 | Real time RT-PCR | [50], Additional file 1 |
| USUV | Real time RT-PCR | [51], Additional file 1 |
| Influenza A virus | Real time RT-PCR | [52], Additional file 1 |
| HAV | Real time RT-PCR | [53], Additional file 1 |
| HEV | Real time RT-PCR | [54, 55], Additional file 1 |
| Norovirus genogroup I (GI) and II (GII) | Real time RT-PCR | [53–55], Additional file 1 |
| DNA Virus | PCR Protocol | References |
| Herpesvirus | nested PCR | [56], Additional file 1 |
| Orthopoxvirus (OPV) | SYBR Green real time PCR | [57], Additional file 1 |
| Parvovirus | Quanty® Parvo B19 kit | Additional file 1 |