Fig. 7.

U0126 and G15 reversed the effect of ICT on the phenotypic polarization of microglia M1/M2 in tMCAO rats. A and B Representative immunofluorescent images of microglia polarization in the ischemic penumbra. The immunofluorescence staining of microglia with anti-CD68 (magenta), CD206 (magenta), and Iba1 (green) antibody. The nucleus was stained by DAPI; A: scale bar = 10 μm; B: scale bar = 50 μm. C–E Immunofluorescence quantitation (n = 3). F–H The protein expressions of CD40 and CD206 (n = 4). The results are expressed as the mean ± SD, *P < 0.05, **P < 0.01, ****P < 0.0001, compared with tMCAO + ICT group