Table 1.
Details of qPCR primers
| Gene abbreviation | Forward sequence | Reverse sequence |
|---|---|---|
| GAPDH | GGGTGTGAACCACGAGAAAT | ACTGTGGTCATGAGCCCTTC |
| SREBP-1c | GGAGCCATGGATTGCACATT | AGGCCAGGGAAGTCACTGTCT |
| FBP1 | CCATCATAATAGAGCCCGAGAAGA | CTTTCTCCGAAGCCTCATTAGC |
| PI3K | ATGCAACTGCCTTGCACATT | ACTGGCAATCTCAGCTGCC |
| AKT | GCCCAACACCTTCATCATCC | GTCTCCTCCTCCTGCCGTTT |
| FoxO1 | TCCTCGAACCAGCTCAAACG | GGCGGTGCAAATGAATAGCAAG |
| CRTC2 | AGGCCTGCTTAGTACAGCCCT | GGCCACTCACTGATCCCTCA |
| CBP | TAATGGAGGCTGCCCAGTGTGTAA | CTGGCGGAGCTTGTGTTTGATGTT |
| mTOR | GTGTGGCAAGAGCGGCAGAC | TGTTGGCAGAGGATGGTCAAGTTG |
| P70S6K | CAGACTCCACCAATCCACAGCAC | TTGCGTGTGCGAAGGAAGAACC |
| AMPKα1 | TTGCGTGTGCGAAGGAAGAACC | CCGATCTCTGTGGAGTAGCAGTCC |
| AMPKα2 | ATGATGAGGTGGTGGAGCAGAGG | GTTCTCGGCTGTGCTGGAATCG |
| FAS | ATGGCTGTCCTGCCTCTGGT | ACGCTCCTCTTCAACTCCAAA |
| ACC | GAGATTTCACTGTGGCTTCGC | TAATTGTTGTTGTTTGCTCCTCC |
| SIRT1 | TGACCTCCTCATTGTTATTGG | GGCATACTCGCCACCTAA |
| CREB | CTGATTCCCAAAAACGAAGG | CTGCCCACTGCTAGTTTGGT |
| PCK1 | CAGGATCGAAAGCAAGACGGT | AAGTCCTCTTCTGACATCCAG |
| G6PC | GACTGGTTCAACCTCGTCTTT | GTCTCACAGGTGACGGGGAAC |
| PPAR-a | ACAAGGCCTCAGGATACCA | GCCGAAAGAAGCCCTTGCAG |
| GLUT2 | AGACAACAACTCCGCACG | TCCAGAGGAACACCCAAAAC |
| GCK | GTGTACAAGCTGCACCCGA | CAGCATGCAAGCCTTCTTG |
| Chrebp | ACAAGAAGCGGCTCCGTAAGTCC | GGGGGCGATAATTGGTGAAGAAA |